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Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

《环境科学与工程前沿（英文）》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1274-5

摘要： Abstract • Sampling parameters with high efficiency was determined. • Operational process to detect airborne ARGs was optimized. • Providing research basis to control airborne ARGs of a laboratory atmosphere Antibiotic resistance genes (ARGs) have been detected in various atmospheric environments. Airborne ARGs transmission presents the public health threat. However, it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples. In this study, an optimized protocol for collecting and detecting airborne ARGs was presented. Experimental results showed that recovery efficiency tended to increase initially and then declined over time, and a range of 550–780 copies/mm2 of capture loading was recommended to ensure that the recovery efficiency is greater than 75%. As the cell walls were mechanically disrupted and nucleic acids were released, the buffer wash protects ARGs dissolution. Three ratios of buffer volume to membrane area in buffer wash were compared. The highest concentrations of airborne ARGs were detected with 1.4 µL/mm2 buffer wash. Furthermore, the majority of the cells were disrupted by an ultrasonication pretreatment (5 min), allowing the efficiency ARGs detection of airborne samples. While, extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments. Therefore, this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments. An optimized sampling method was proposed that the buffer wash was 1.4 µL/mm2 and the ultrasonication duration was 5 min. The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories. The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

关键词： Airborne tetracycline resistance genes     Filter sampling     Capture loading     Membrane pretreatment     Indoor laboratory atmosphere    

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Numerical simulation and experimental research on the wheel brush sampling process of an asteroid sampler

《机械工程前沿（英文）》 2023年 第18卷 第2期 doi: 10.1007/s11465-022-0732-0

摘要： To examine the environmental characteristics of the microgravity force and the weathered layer on an asteroid surface, a symmetric wheel brush asteroid sampler is proposed for the collection of particles on the asteroid surface. To study the influence of the wheel brush rotation speed on the sampling efficiency and the driving torque required for the wheel brush, the contact dynamics model between particles and sampling wheel brushes is established and a simulation and experimental verification of the sampling process are conducted. The parameter calibration of the sampled particles is studied first, and the calibrated particle parameters are used in the numerical simulation of the sampling process. The sampling results and the particle stream curves are obtained for the working conditions of different rotation speeds, and the effects of different parameter settings on the sampling efficiency are analyzed. In addition, a set of rotating symmetrical sampling wheel brush devices is built for the ground test, and the dynamic torque sensor is used to test the torque change of the wheel brush during the sampling process. The relationship between the speed of the wheel brush and the driving torque of the wheel brush motor is determined by comparing the simulation results with the test results. Results indicate that when the rotating speed of the wheel brush is faster, the sampling efficiency is higher, and the driving torque required for the sampling wheel brush is greater. Moreover, a numerical simulation analysis of the sampling process of the wheel brush sampler in a microgravity environment is conducted to determine the optimal speed condition, and the brushing test of the wheel brush sampler in the microgravity environment is verified with the drop tower method. This research proposes the structural optimization design and motor selection of a wheel brush asteroid sampler, which provides important reference value and engineering significance.

关键词： asteroid sampling     wheel brush sampler     discrete element method     parameter calibration     experimental research    

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Investigation on sampling artifacts of particle associated PAHs using ozone denuder systems

Kai LIU, Fengkui DUAN, Kebin HE, Yongliang MA, Yuan CHENG

《环境科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 284-292 doi: 10.1007/s11783-013-0555-7

摘要： Polycyclic aromatic hydrocarbons (PAHs) are complex organic compounds which are identified as significant carcinogenic to human health. PAHs (mainly in particle phase) are susceptible to atmospheric oxidant gases, especially ozone, nitrogen oxides (NO ), hydroxyl radical (OH), and could be degraded on filters during sampling process, leading to an underestimation of ambient PAH concentrations. The goal of this work was to investigate particle associated PAHs sampling artifacts caused by ozone in summer of Beijing. Comparative sampling systems were operated simultaneously during the whole campaign, one with activated carbon ozone denuder, the other being set as conventional sampling system. Activated carbon denuder was testified to be highly efficient to eliminate ozone from air stream. In general, nine particle-bound PAHs observed from conventional sampler were all lower than those from ozone denuder system. The total PAHs (particle phase) concentration was averagely underestimated by 35.9% in conventional sampling procedure. Benzo[a]pyrene (BaP) had the highest percentage of mass loss. Ambient temperature was founded to have influences on PAHs sampling artifacts. High temperature can increase loss of particle associated PAHs during sampling.

关键词： particle associated polycyclic aromatic hydrocarbons (PAHs)     ozone     denuder     sampling artifacts    

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A sampling method based on URL clustering for fast web accessibility evaluation

Meng-ni ZHANG,Can WANG,Jia-jun BU,Zhi YU,Yu ZHOU,Chun CHEN

《信息与电子工程前沿（英文）》 2015年 第16卷 第6期   页码 449-456 doi: 10.1631/FITEE.1400377

摘要： When evaluating the accessibility of a large website, we rely on sampling methods to reduce the cost of evaluation. This may lead to a biased evaluation when the distribution of checkpoint violations in a website is skewed and the selected samples do not provide a good representation of the entire website. To improve sampling quality, stratified sampling methods first cluster web pages in a site and then draw samples from each cluster. In existing stratified sampling methods, however, all the pages in a website need to be analyzed for clustering, causing huge I/O and computation costs. To address this issue, we propose a novel page sampling method based on URL clustering for web accessibility evaluation, namely URLSamp. Using only the URL information for stratified page sampling, URLSamp can efficiently scale to large websites. Meanwhile, by exploiting similarities in URL patterns, URLSamp cluster pages by their generating scripts and can thus effectively detect accessibility problems from web page templates. We use a data set of 45 web sites to validate our method. Experimental results show that our URLSamp method is both effective and efficient for web accessibility evaluation.

关键词： Page sampling     URL clustering     Web accessibility evaluation    

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Dilution sampling and analysis of particulate matter in biomass-derived syngas

Xiaoliang WANG, Curtis ROBBINS, S. Kent HOEKMAN, Judith C. CHOW, John G. WATSON, Dennis SCHUETZLE

《环境科学与工程前沿（英文）》 2011年 第5卷 第3期   页码 320-330 doi: 10.1007/s11783-011-0347-x

摘要： Thermochemical biomass gasification, followed by conversion of the produced syngas to fuels and electrical power, is a promising energy alternative. Real-world characterization of particulate matter (PM) and other contaminants in the syngas is important to minimize damage and ensure efficient operation of the engines it powers and the fuels created from it. A dilution sampling system is demonstrated to quantify PM in syngas generated from two gasification plants utilizing different biomass feedstocks: a BioMax 15 Biopower System that uses raw and torrefied woodchips as feedstocks, and an integrated biorefinery (IBR) that uses rice hulls and woodchips as feedstocks. PM mass concentrations in syngas from the IBR downstream of the purification system were 12.8–13.7 μg·m , which were significantly lower than the maximum level for catalyst protection (500 μg·m ) and were 2–3 orders of magnitude lower than those in BioMax 15 syngas (2247–4835 μg·m ). Ultrafine particle number concentration and PM chemical constituents were also much lower in the IBR syngas than in the BioMax 15. The dilution sampling system enabled reliable measurements over a wide range of concentrations: the use of high sensitivity instruments allowed measurement at very low concentrations (~1 μg·m ), while the flexibility of dilution minimized sampling problems that are commonly encountered due to high levels of tars in raw syngas (~1 g·m ).

关键词： dilution source sampling     syngas characterization     biomass gasification     ultrafine particles    

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Implicit Heaviside filter with high continuity based on suitably graded THB splines

《机械工程前沿（英文）》 2022年 第17卷 第1期   页码 14-14 doi: 10.1007/s11465-021-0670-2

摘要： The variable density topology optimization (TO) method has been applied to various engineering fields because it can effectively and efficiently generate the conceptual design for engineering structures. However, it suffers from the problem of low continuity resulting from the discreteness of both design variables and explicit Heaviside filter. In this paper, an implicit Heaviside filter with high continuity is introduced to generate black and white designs for TO where the design space is parameterized by suitably graded truncated hierarchical B-splines (THB). In this approach, the fixed analysis mesh of isogeometric analysis is decoupled from the design mesh, whose adaptivity is implemented by truncated hierarchical B-spline subjected to an admissible requirement. Through the intrinsic local support and high continuity of THB basis, an implicit adaptively adjusted Heaviside filter is obtained to remove the checkboard patterns and generate black and white designs. Threefold advantages are attained in the proposed filter: a) The connection between analysis mesh and adaptive design mesh is easily established compared with the traditional adaptive TO method using nodal density; b) the efficiency in updating design variables is remarkably improved than the traditional implicit sensitivity filter based on B-splines under successive global refinement; and c) the generated black and white designs are preliminarily compatible with current commercial computer aided design system. Several numerical examples are used to verify the effectiveness of the proposed implicit Heaviside filter in compliance and compliant mechanism as well as heat conduction TO problems.

关键词： topology optimization     truncated hierarchical B-spline     isogeometric analysis     black and white designs     Heaviside filter    

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Unified methodology for site-characterization and sampling of highway runoff

Jy S. WU, Craig J. ALLAN,

《环境科学与工程前沿（英文）》 2010年 第4卷 第1期   页码 47-58 doi: 10.1007/s11783-010-0003-x

摘要： Hydrology, roadway traffic conditions, and atmospheric deposition are three essential data categories for the planning and implementation of highway-runoff monitoring and characterization programs. Causal variables pertaining to each data category could be site specific but have been shown to correlate with runoff pollutant loads. These data categories were combined to derive statistical relationships for characterization and prioritization of the respective pollutant loads at highway runoff sites. Storm runoff data of total suspended solids (TSS), total dissolved solid (TDS), chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN) and total phosphorus (TP) collected from three highway sites in Charlotte, North Carolina, USA, were used to illustrate the development of site-specific highway-runoff pollutant loading models. This unified methodology provides a basis for initial assessment of the pollutant-constituent loads from highway runoff using hydrologic component variables. Improved reliability is achievable when additional traffic and/or atmospheric component variables are incorporated into the basic hydrologic regression model. In addition, operational guidance is suggested for implementing highway-runoff monitoring programs that are subject to sampling and resources constraints.

关键词： highway runoff     pollutant loads     regression models     non-point source pollution     storm water permit    

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Anovel approach of noise statistics estimate using H∞ filter in target tracking

Xie WANG,Mei-qin LIU,Zhen FAN,Sen-lin ZHANG

《信息与电子工程前沿（英文）》 2016年 第17卷 第5期   页码 449-457 doi: 10.1631/FITEE.1500262

摘要： Noise statistics are essential for estimation performance. In practical situations, however, a priori information of noise statistics is often imperfect. Previous work on noise statistics identification in linear systems still requires initial prior knowledge of the noise. A novel approach is presented in this paper to solve this paradox. First, we apply the H∞ filter to obtain the system state estimates without the common assumptions about the noise in conventional adaptive filters. Then by applying state estimates obtained from the H∞ filter, better estimates of the noise mean and covariance can be achieved, which can improve the performance of estimation. The proposed approach makes the best use of the system knowledge without a priori information with modest computation cost, which makes it possible to be applied online. Finally, numerical examples are presented to show the efficiency of this approach.

关键词： Noise estimate     H∞     filter     Target tracking    

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Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

《环境科学与工程前沿（英文）》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1080-5

摘要：

• An ibuprofen-degrading strain, Serratia marcescens BL1, was isolated and identified.

• The effects of various factors on ibuprofen degradation by BL1 were evaluated.

• Strain BL1 was applied to a laboratory-scale biological aerated filter system.

• Strain BL1 was stable in both static tests and in the biological aerated filter system.

关键词： Ibuprofen     Biological aerated filter     Degrading bacterium     Serratia marcescens    

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Uncertainty propagation in dynamics of composite plates: A semi-analytical non-sampling-based approach

Mahdi FAKOOR, Hadi PARVIZ

《结构与土木工程前沿（英文）》 2020年 第14卷 第6期   页码 1359-1371 doi: 10.1007/s11709-020-0658-8

摘要： In this study, the influences of spatially varying stochastic properties on free vibration analysis of composite plates were investigated via development of a new approach named the deterministic-stochastic Galerkin-based semi-analytical method. The material properties including tensile modulus, shear modulus, and density of the plate were assumed to be spatially varying and uncertain. Gaussian fields with first-order Markov kernels were utilized to define the aforementioned material properties. The stochastic fields were decomposed via application of the Karhunen-Loeve theorem. A first-order shear deformation theory was assumed, following which the displacement field was defined using admissible trigonometric modes to derive the potential and kinetic energies. The stochastic equations of motion of the plate were obtained using the variational principle. The deterministic-stochastic Galerkin-based method was utilized to find the probability space of natural frequencies, and the corresponding mode shapes of the plate were determined using a polynomial chaos approach. The proposed method significantly reduced the size of the mathematical models of the structure, which is very useful for enhancing the computational efficiency of stochastic simulations. The methodology was verified using a stochastic finite element method and the available results in literature. The sensitivity of natural frequencies and corresponding mode shapes due to the uncertainty of material properties was investigated, and the results indicated that the higher-order modes are more sensitive to uncertainty propagation in spatially varying properties.

关键词： composite plate     spatially varying stochastic properties     Galerkin method     polynomial chaos approach     semi-analytical approach    

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Rapid transaction to load variations of active filter supplied by PV system

M. BENADJA,S. SAAD,A. BELHAMRA

《能源前沿（英文）》 2014年 第8卷 第3期   页码 335-344 doi: 10.1007/s11708-014-0325-7

摘要： This paper deals with the analysis and control of a photovoltaic (PV) system connected to the main supply through a Boost converter and shunt active filter supplied by a PV system providing continuous supply of nonlinear load in variation. A robust control of a PV system connected to the grid while feeding a variable nonlinear load is developed and highlighted. This development is based on the control of the Boost converter to extract the maximum power from the PV system using the Perturb and Observe (P and O) algorithm in the presence of temperature and illumination. The proposed modeling and control strategy provide power to the variable nonlinear load and facilitates the transfer of power from solar panel to the grid while improving the quality of energy (harmonic currents compensation, power factor compensation and dc bus voltage regulation). Validation of the developed model and control strategy is conducted using power system simulator Sim-Power System Blockset Matlab/Simulink. To demonstrate the effectiveness of the shunt active filter to load changes, the method of instantaneous power ( theory) is used to identify harmonic currents. The obtained results show an accurate extraction of harmonic currents and perfect compensation of both reactive power and harmonic currents with a lower THD and in accordance with the IEEE-519 standard.

关键词： solar panels     maximum power point tracking (MPPT)     DC/DC converter (Boost)     shunt active filter     instantaneous power control     power quality     harmonics     imbalances     reactive energy    

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Effect of biological activated carbon filter depth and backwashing process on transformation of biofilm

Wanqi Qi, Weiying Li, Junpeng Zhang, Xuan Wu, Jie Zhang, Wei Zhang

《环境科学与工程前沿（英文）》 2019年 第13卷 第1期 doi: 10.1007/s11783-019-1100-0

摘要：

We studied BAC biofilm during the process of initial operation and backwash.

Microbial diversity decreased gradually with the increase of BAC filter depth.

Proteobacteria dominated at the phylum level among the BAC biofilm samples.

α-proteobacteria increased about 10% in all carbon filter depth after backwash.

关键词： Biological activated carbon     Biofilm     Community structure     Carbon filter depth     High-throughput sequencing    

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Ceramic water filter for point-of-use water treatment in developing countries: Principles, challenges

Haiyan Yang, Shangping Xu, Derek E. Chitwood, Yin Wang

《环境科学与工程前沿（英文）》 2020年 第14卷 第5期 doi: 10.1007/s11783-020-1254-9

摘要： • CWF is a sustainable POU water treatment method for developing areas. • CWF manufacturing process is critical for its filtration performance. • Simultaneous increase of flow rate and pathogen removal is a challenge. • Control of pore size distribution holds promises to improve CWF efficiency. • Novel coatings of CWFs are a promising method to improve contaminant removal. Drinking water source contamination poses a great threat to human health in developing countries. Point-of-use (POU) water treatment techniques, which improve drinking water quality at the household level, offer an affordable and convenient way to obtain safe drinking water and thus can reduce the outbreaks of waterborne diseases. Ceramic water filters (CWFs), fabricated from locally sourced materials and manufactured by local labor, are one of the most socially acceptable POU water treatment technologies because of their effectiveness, low-cost and ease of use. This review concisely summarizes the critical factors that influence the performance of CWFs, including (1) CWF manufacturing process (raw material selection, firing process, silver impregnation), and (2) source water quality. Then, an in-depth discussion is presented with emphasis on key research efforts to address two major challenges of conventional CWFs, including (1) simultaneous increase of filter flow rate and bacterial removal efficiency, and (2) removal of various concerning pollutants, such as viruses and metal(loid)s. To promote the application of CWFs, future research directions can focus on: (1) investigation of pore size distribution and pore structure to achieve higher flow rates and effective pathogen removal by elucidating pathogen transport in porous ceramic and adjusting manufacture parameters; and (2) exploration of new surface modification approaches with enhanced interaction between a variety of contaminants and ceramic surfaces.

关键词： Point-of-use water treatment     Ceramic water filter     Bacterial removal     Surface modification     Water quality    

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亚奈奎斯特采样的数据恢复：性能极限与恢复算法

罗茜倩,张朝阳

《信息与电子工程前沿（英文）》 2021年 第22卷 第2期   页码 141-286 doi: 10.1631/FITEE.1900320

摘要： 奈奎斯特频率是一般带限信号进行无损采样的采样率下界，然而在某些情景中，亚奈奎斯特频率也足以进行无损采样和信号恢复。以往对亚奈奎斯特采样的研究主要集中在利用信号变换来降低信号维度，但是亚奈奎斯特采样信号的结构并没有得到充分研究。本文针对线性调制基带信号的亚奈奎斯特采样，研究其信号恢复性能极限与算法。该问题中，原信号维度无法降低，因此亚奈奎斯特采样不可避免会带来信息损失，信号恢复也变成一个欠定线性问题。本文采用两种亚奈奎斯特采样方法对线性调制基带信号进行采样，分别研究了两种采样方法下的性能极限和信号恢复算法。首先，针对两种亚奈奎斯特采样方法，分别计算了采样序列之间的最小归一化欧氏距离，以此作为最优性能的指标。然后，在基带信号有限符号集的限制条件下，采用改进的时变维特比算法从亚奈奎斯特采样序列中恢复原信号。将仿真得到的亚奈奎斯特采样的误比特率与其性能的理论极限比较，并与奈奎斯特采样性能对比，验证了时变维特比算法在信号恢复问题中的优良性能。

关键词： 奈奎斯特采样定理；亚奈奎斯特采样；最小欧式距离；欠定线性问题；时变维特比算法    

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Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated Filter

Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG

《环境科学与工程前沿（英文）》 2011年 第5卷 第2期   页码 291-297 doi: 10.1007/s11783-010-0270-6

摘要： Shortcut nitrification-denitrification, anaerobic ammonium oxidation (ANAMMOX), and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system. As fed different synthetic wastewater with chemical oxygen demand (COD) of 300–1200 mg·L and of 30–120 mg·L at the outer recycle ratio of 200%, the influence of influent on ANAMMOX in the integrated system was investigated in this paper. The experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX; however, when an influent with the low concentration of COD was used, the opposite changes could be observed. Higher influent concentration favored ANAMMOX when the COD concentration of influent was fixed. Therefore, low ratio would decrease competition for nitrite between ANAMMOX and denitrification, which was favorable for reducing the negative effect of organic COD on ANAMMOX. The good performance of the integrated system indicated that the bacterial community of denitrification, ANAMMOX, and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.

关键词： Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system     the influence of influent     anaerobic ammonium oxidation (ANAMMOX)     shortcut nitrification-denitrification     methano-genesis    

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标题 作者 时间 类型 操作

Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

期刊论文

Numerical simulation and experimental research on the wheel brush sampling process of an asteroid sampler

期刊论文

Investigation on sampling artifacts of particle associated PAHs using ozone denuder systems

Kai LIU, Fengkui DUAN, Kebin HE, Yongliang MA, Yuan CHENG

期刊论文

A sampling method based on URL clustering for fast web accessibility evaluation

Meng-ni ZHANG,Can WANG,Jia-jun BU,Zhi YU,Yu ZHOU,Chun CHEN

期刊论文

Dilution sampling and analysis of particulate matter in biomass-derived syngas

Xiaoliang WANG, Curtis ROBBINS, S. Kent HOEKMAN, Judith C. CHOW, John G. WATSON, Dennis SCHUETZLE

期刊论文

Implicit Heaviside filter with high continuity based on suitably graded THB splines

期刊论文

Unified methodology for site-characterization and sampling of highway runoff

Jy S. WU, Craig J. ALLAN,

期刊论文

Anovel approach of noise statistics estimate using H∞ filter in target tracking

Xie WANG,Mei-qin LIU,Zhen FAN,Sen-lin ZHANG

期刊论文

Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

期刊论文

Uncertainty propagation in dynamics of composite plates: A semi-analytical non-sampling-based approach

Mahdi FAKOOR, Hadi PARVIZ

期刊论文

Rapid transaction to load variations of active filter supplied by PV system

M. BENADJA,S. SAAD,A. BELHAMRA

期刊论文

Effect of biological activated carbon filter depth and backwashing process on transformation of biofilm

Wanqi Qi, Weiying Li, Junpeng Zhang, Xuan Wu, Jie Zhang, Wei Zhang

期刊论文

Ceramic water filter for point-of-use water treatment in developing countries: Principles, challenges

Haiyan Yang, Shangping Xu, Derek E. Chitwood, Yin Wang

期刊论文

亚奈奎斯特采样的数据恢复：性能极限与恢复算法

罗茜倩,张朝阳

期刊论文

Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated Filter

Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG

期刊论文